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The N-terminal domain, containing eight PPPVHL repeats and a Pro-X sequence, allows ER retention and accumulation of fusion proteins in membrane-defined protein bodies, and may also determine interaction with membranes.
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Surface properties such as physicochemical characteristics and topographical parameters are important considerations in the design of implant materials, as they determine interactions with living cells and tissues.
To determine interactions with β-dystroglycan, 100 mg of skeletal muscle was solubulized in 1 mL of 1% digitonin solution (1% Digitonin, 50 mM Tris HCl, 500 mM NaCl, and protease inhibitor [Roche catalogue number 4693116]).
We treated cells expressing either FLAG-CUL3WT or FLAG- CUL3Δ403 459 with the neddylation inhibitor MLN4924 to force all of CUL3 into the non-neddylated state and then determined interaction with CAND1 by immunoprecipitation.
Our data show how extracellular signals initially converted to kinase signalling connect to arginine methylation of a transcription factor that in turn determines interaction with the epigenetic and gene regulatory machinery in the nucleus to regulate gene transcription and differentiation.
To determine interaction of TIMP2 with membrane-associated MT1-MMP, we used lysates made from WT and CD44−/− osteoclasts surface labeled with NHS-biotin.
The standard mixing rule σ = (σ1 + σ2)/2 was used to determine interactions between particles with different diameters σ1 and σ2.
The relative concentrations of acetylated and non-acetylation survivin at lysine 129 determine its interaction with CRM1, and thus regulate the nuclear export of survivin [ 75].
All things look so different and have different colours because of their different sets of internal discrete energies, which determine their interaction with electromagnetic radiation.
Accordingly, it could be hypothesized that the surface proteomic signature of a biomaterial might determine its interaction with cells.
Since the unique loop structures of aptamers determine their interaction with targets, encoding their central loop structures directly enables featuring aptamer binding affinity related properties.
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