To determine how ERK signaling effects mRNA transcription during wound healing, we also collected U0126-treated animRNAmRNA one and four hours after injury for comparison.
To determine how this mutation affects mRNA splicing, we used RT-PCR to amplify RNA from rad3-1 mushroom caps and then cloned the PCR products.
An interesting area for future research will be to determine how specific types of mRNAs are recognized and targeted for these quality control systems.
In order to determine how fast kinetin modulates IKBKAP mRNA splicing, we performed a time-course experiment with a constant concentration of 80 µM over 24 h.
Although our aim in this study was to examine RNA decay, we were able to use our data to first determine how OA affects steady-state mRNA levels, using the zero time point of our decay curves.
To study the impact of inducing these different cellular states in the yeast chemostat cultures, transcription profiling by RNAseq was used to determine how gene expression at the mRNA level changed following induction of the T70N and I56T variants with respect to the control strain.
To determine how CBA affects the expression of mRNA at end-stage SIV infection, total RNA was isolated from SKM at necropsy from a total of 28 animals (SUC/SIV (n = 9), CBA/SIV (n = 11), and control (n = 8)) and microarray analysis was performed as previously described on all samples [ 13].
Further work is required to determine how specific variants affect levels of messenger RNA (mRNA) and consequently protein, which could provide further insight into mechanisms of pathogenesis.
Exon-level data can be used to investigate splicing events, which determine how individual exons are joined together to form a mature mRNA transcript.
To determine how accurately SAGE identified germline-enriched genes, we analyzed the mRNA in situ expression patterns of these 603 genes using the NEXTDB database.
