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Contigs from de novo assemblies were aligned to the reference genome using BLAST in order to determine gap positions.
To determine gap junction activity, recorded images were analyzed for the density of calcein dye uptake by unlabeled cells and calcein retained by DiD-labeled donor cells using ImageJ.
Such a representation also makes it easier to determine gap thresholds for phylogenetic accuracy in empirical studies, to determine the expected level of accuracy given a certain percentage of gaps in an alignment.
Neighborhood sequence information has been used by many programs [such as PSIPRED (McGuffin et al., 2000)] for protein local structure prediction and by a few for protein sequence alignment and homology search (Biegert and Söding, 2009), but such information has not been applied to protein threading, especially to determine gap opening.
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This instrument will be useful in assessing pain management knowledge of clinical nurses to determine gaps in education, evaluate knowledge after pain management education, and measure research outcomes.
For instance, we could use a grid to assess coverage of a map such as that shown in Fig. 3, and determine gaps where coverage is less than that desired for individual cells.
In February 2003, the National Institute of Child Health and Human Development NICHDD) and the US Food and Drug Administration FDAA) created the Newborn Drug Development Initiative (NDDI), an ongoing program to determine gaps in knowledge in neonatal therapeutics and to explore clinical study designs for use in the newborn population.
Our first goal is to analyze spatial heterogeneity of the data to determine gaps in knowledge and the effect of biases in the distribution of geographical data within the established ecoregional biogeographical divisions in the Caribbean.
To determine gap-junction-dependent dye diffusion, cells were grown on a coverslip coated with Geltrex and cultured in neurobasal media with B27, EGF, and bFGF.
Finally, a microinjection/dye-transfer assay, employed to determine gap-junction functionality, clearly demonstrated that the cells were functionally coupled, albeit to varying degrees, in differentiated and undifferentiated phenotypes.
Unmet need is a standard measure used to determine gaps in FP services and is a key indicator of the FP2020 initiative [ 18].
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