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We therefore set out to determine expression of the target genes that we have identified.
To confirm this hypothesis, RT-PCR analysis was performed to determine expression of the early mesodermal marker (BrachyuryT and Tbx6), cardiomyocyte-specific transcription factors (Csx/Nkx2.5), structural genes (β-MyHC), and Gapdh (Fig. 4A).
Immunohistochemical staining (IHC) was performed to determine expression of the ER and progesterone receptor (PR).
Total RNA was isolated from the brain, spinal cord and thymus of transgenic mice to determine expression of the transgenes.
The ΔΔCt method was used to determine expression of the gene of interest, and the expression of effector cells were set equal to 1 [ 39].
Therefore, we sought to determine expression of the rat genes Mmp2, Mmp9, Timp1 and Timp2 in the liver of CCl4-treated rats with different degrees of cirrhosis.
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Next, we determined expression of the Sulfs in human pancreatic adenocarcinoma cell lines by using RT-PCR to survey a panel of 24 cell lines.
We also determined expression of the epithelial and mesenchymal cell markers, cytokeratin 8 (CK8) and vimentin, respectively.
In this study, we determined expression of the total leptin receptor (ObR) as well as ObRb.
Determining expression of the calpain system may be of benefit in patients with pancreatic, bile duct of ampullary cancers.
We first determined expression of the myc-tagged mutant forms using western blot analysis of embryo lysates.
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