(F) TaqMan qPCR was performed to determine expression of mRNA expression of indicated genes.
(C) Semi-quantitative real-time PCR to determine expression of inflammatory mediators in WT and Ninj1 KO MLE-12.
To determine expression of stanniocalcin-1 (STC1) in human endometrium with and without endometriosis and its regulation by steroid hormones.
Reverse transcriptase-polymerase chain reaction, Western analysis, and immunohistochemistry were used to determine expression and localization of AQP1 and AQP3.
Through scRNA-seq researchers are able to determine expression profiles in single-cell resolution.
Post-differentiation, flow cytometry was used to determine expression of CD71 and CD235a (Glycophorin-A).
To determine expression levels of the genes, iWAT was acutely dissected from the mice at indicated time points after treatment.
Spinelli et al. used a candidate gene approach to determine expression of six miRNAs in PBMCs of TB patients and TSTneg individuals [14].
Genevestigator (Grennan 2006), a widely used resource for investigating spatio-temporal and response patterns of genes was used to determine expression characteristics of gene sets assigned to specific pathways.
To determine expression pattern of TCD10 gene in various tissues, we analyzed the TCD10 expression level of the root, stems, young-leaf, flag-leaf and panicle in wild-type plant tissues.
In this context, we would like to caution that, as usual, antibody-dependent methods to determine expression levels depend on the antibody-specificity of the antibodies used and thus need to be received with the necessary prudence.
