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HPV-6 and HPV-11 amplicons were used to determine detection limits and inter- and intra-assay variability.
To determine detection performance properties, avalanche pulses were collected from each device, discriminated at 50 mV threshold and time-tagged with a resolution of 156.25 ps, while applied bias voltages and thermal parameters were simultaneously recorded at 10 Hz.
Detection limits are considered key parameters to determine detection capability and applicability of biosensing tools.
However, research is currently ongoing to determine detection limits and detection frequencies using this method.
To determine detection limits of the assay, plasmids containing the amplified regions of groEL (O.
This study utilized the adjoint method to determine detection sensitivity profiles for an optical collection fiber.
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In total, 197 single-nucleotide polymorphisms (SNPs) were detected at population frequency > 0.1% (Table S1), the empirically determined detection limit of this sequencing technology.
The determined detection limit and sorption capacity were 0.11 μg/L and 184.50 mg/g, respectively.
A table of the determined detection limits for all species is provided in Table S-2 (Supporting Information).
The thresholds for determining detection calls, the default set by Affymetrix, were the same for all samples.
The determined detection limits of procedure based on the novel fiber enable its application at the concentration levels of triazines recommended by the US EPA standards.
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