Sentence examples for determine copies from inspiring English sources

Taqman real-time PCR was performed on each sample for both the CMV promoter in order to determine copies of the viral genome, and the mouse titin gene, to standardize for number of mouse genomes present in each sample.

Quantitative real time RT-PCR was used to quantify HDV total RNAs, strand-specific real time RT-PCR to determine copies of HDV genome and antigenome RNA (see below).

However, this single-pot strategy requires in silico masking of the coding sequences (exonic region) of the genome to determine copy number variation, which leads to incomplete transcripts from the cell.

In order to determine copy number changes, CGH-Explorer v. 3.1b was used [16].

We sequenced the tumor and normal genomes of one patient and used these data to determine copy number changes (CNCs) between the two samples.

First, we analyzed the CGH loci to determine copy number of whole chromosomes.

Fluorescent in situ hybridisation analysis was used to determine copy number changes of the EGFR gene.

The delta delta Ct method was used to determine copy number.

VNTRs <5 bp were sequenced to determine copy number, and those >5 bp were determined by fragment analysis.

We hypothesized that qPCR could finitely determine copy number and through this process, assess the validity of a calling algorithm.

Raw data was analyzed with StepOne Software v2.2.1 to determine copy number based on the threshold cycles (Ct).