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SRB assay was performed to determine cell viability [20].
Using a microplate reader, absorbance was recorded at 570 nm to determine cell survival.
To determine cell viability by FCM, double staining was performed accordingly to (Hewitt et al. 1999).
To determine cell viability, an MTT assay was conducted according to Mosmann et al.
MTS is a colorometric probe used to determine cell viability and was analyzed using Molecular Devices SpectraMax 340PC3844.
Then, we performed CCK8 assays to determine cell proliferation ability after transfection with control siRNA or CDK6 siRNA.
MTT assay is a rapid colorimetric approach that widely used to determine cell growth and cell cytotoxicity.
Propidium Iodide (PI) is a DNA staining dye and it is widely used to determine cell viability by flow cytometry.
Cell experiments were carried out with PC3 cells to determine cell uptake, efflux and in vitro binding affinity.
A 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine cell viability.
In this study, in order to determine cell density of pure ABS and APCN foams, SEM tests were conducted.
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