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An alternative confirmatory HIV testing strategy based on initial testing on either SD Bioline or Determine assays followed by testing of reactive samples on the Determine or SD Bioline gave 100% sensitivity (95% CI; 99.1 100) and 100% specificity (95% CI; 96 99.1) with Uni-Gold™ as tiebreaker for discordant results.
An alternative confirmatory HIV testing strategy based on initial testing on either SD Bioline or Determine assays followed by testing of reactive samples on the Determine or SD Bioline gave 100% sensitivity (95% CI; 99.1 100) and 100% specificity (95% CI; 96 99.1) with Uni-Gold™ assay as tie breaker for discordant results.
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Characteristics of the patients (n = 11) whose sera were used to determine assay specificity is shown in Table 1.
Lastly, a test sample collection was assayed to determine assay accuracy and precision.
As a control to determine assay background, assay buffer was added instead of microsomes.
and 4 known negative control serum samples were used to determine assay cutoff.
Moxifloxacin (Avelox®; Bayer) [21] was included as the positive control to determine assay sensitivity [22].
> -wrap-foot> limitimit of detection, LOQ limit of quantification Coefficients of variation (RSD, %) were used to determine assay precision.
All markers were submitted through the Illumina Design Tool to determine assay design scores for each marker.
AP participated in carrying out the bacteriophage phage display cultures and purification procedures, carried out phage activity determining assays.
BO and AK carried out LPS analysis in purified preparations and carried out phage activity determining assays.
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