The focus is now swinging toward enumerating individual lipid species, determining their identity, and quantitating their amount.
The ability of a chromatographic method to successful separate, identify and quantitate species is determined by many factors, many of which are in the control of the experimenter.
Absorbance at 550 nm(Cy3) and 650 nm (Cy5) was determined to quantitate incorporation of label, so that equimolar amounts of Cy3 and Cy5 could be used for hybridization.
The copy numbers of the standard template were determined to quantitate ID-1, ID-2 and ID-3 mRNA level in samples for real-time RT PCR.
The copy numbers of the standard template were determined to quantitate PAR-2 mRNA level in samples for real-time reverse transcription (RT -PCR.
Sensors are used to detect and quantitate clinically relevant analytes.
Cell viability was determined by WST-1 anday and quantitated spectrophotometrically by measuring the optical density at 450 nm, with a reference wavelength of 650 nm.
The presence of SHEAR deformity was determined by physical examination and quantitated from 3D-CT images [ 4].
Initial reactivity data on 9 HBsAg assay configurations determined for 28 defined and quantitated HBsAg recombinant mutant antigens (29 ) have been confirmed by several groups.
