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"Most people thought this was too far out in the future, to actually determine a structure," Dr. MacKinnon said.
The model obtained was used to determine a structure solution for the 2.8-Å data.
It is quite rare these days to determine a structure of a soluble protein and really find something new.
To determine a structure with the intact donor substrate bound, the predicted nucleophilic residue, Asp394, was mutated to Ala.
(162) As in protein crystallography, it is usually much quicker and easier to determine a structure if a sufficiently similar starting model is already available.
The TtrD protein was overproduced and purified, and attempts were made to determine a structure of the complex with a TtrA signal peptide.
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These GBRS assign scores to buildings based on a variety of assessment criteria to determine a structures environmental impact.
"Strain is a very important parameter," he says, and often determines a structure's stability and why it grows the way it does.
We obtained crystals of phosphoUb and determined a structure to 1.9 Å resolution (Table 1).
This software compares alignments of the sample protein to other proteins with known structural information to determine a probable structure using statistics.
Also, even with the more efficient techniques, it can still take weeks and cost tens of thousands of dollars to determine a single structure.
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