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Because of the amount of labor involved in obtaining the PhyCoI we suggest to combine low frequency PhyCoI determinations with a high frequency Secchi depth measurements for practical monitoring purposes.
Patch determinations with a molecular viewer of crystalline structural models downloaded from the Entrez Molecular Modeling Database Web site led to the identification of 44 DRB, 33DQB, 29 DQA, 20 DPB, and 9 DPA unique combinations of polymorphic positions.
1) LOD determinations, with a dilution series of all strains, were tested by using real-time RT-PCR (15 ).
Determination of the Ki for CpOGA was performed by steady-state kinetics using the parameters described for CpOGA IC50 determinations with a series of inhibitor concentrations (0 110 μM. The reactions were stopped by the addition of 100 μl 3 M glycine NaOH, pH 10.3. The fluorescence of the released 4MU was quantified as described previously [39,31,40].
The reaction mixture was further incubated at 16 °C for 16 h, followed by incubation at 65 °C for 20 min. After incubation, Probe P was purified on a 12% denaturing PAGE gel (7 M urea, 1× TBE), and its final concentration was confirmed by absorption determinations with a Nanodrop ND-1000 spectrophotometer (Wilmington, DE, USA).
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Modern techniques typically yield age determinations with an estimated error of 2 million years or less, even for rocks of Archean age.
The high signal-to-noise ratio provided by laser absorption provides rate coefficient determinations with an estimated uncertainty of ±20%.
The sensor was tested together with time-dependent sigmoidal calibrations giving pH determinations with an exceptional precision and accuracy during measurement within pH 6 8.
Serum VEGF determinations with an intra-assay variance below 10% were included in the study (CV max; nearly 9%).
This revealed 4661 transcription units (TUs), which was a huge increase over previous determinations, with an average of 1.7 promoters per operon.
A validation study comparing prospectively determined time to pregnancy and a later retrospective determination, with a median recall of 14 years, found 80% sensitivity and 95% specificity for infertility (time to pregnancy of ≥ 12 months) (Joffe et al. 1993).
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