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All determinations were subjected to the ISO-9001-2000 requirements.
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69 proteins that had previously resisted structure determination were subjected to crystallization with in situ proteolysis and ten crystallized in a form that led to structure determination (14.5% success rate).
More than 270 proteins that failed to produce crystals or that produced crystal forms unsuitable for structure determination were subjected to in situ proteolysis crystallization trials over an eight-month period in 2008.
The in vivo method for digestibility determination is subjected to different factors affecting its accuracy.
For dry matter determination, lyophilised subsamples were subjected to a drying procedure at 103°C for at least 4 h.
After initial T max determination, the muscles were subjected to a protocol that mimics normal muscle activity (No fatigue, stimulation trains triggered at every minute, duty cycle of 2%).
The fractions obtained were subjected to determination of antioxidative activities as described above.
Ethanol and methanol extract from both seaweeds without and with chlorophyll removal were subjected to determination of antioxidative activity.
Twenty-four hours after transfection, cells were subjected to determination of luciferase activity as described [17], [19], [32].
The obtained lysates were subjected to determination of enzymatic activity using the colorimetric method detailed above.
After 48 h, cells were subjected to determination of hTERT mRNA and TA.
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