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All determinations were run in triplicate.
All determinations were run in duplicate.
All determinations were run as duplicates and mean values calculated.
Other laboratory determinations were run on fully automated analyzers continuously maintained in readiness to use, undergoing regular service and operated in accordance with manufacturer's recommendations.
Separate isocratic determinations were run for a) DA and NA, b) 5-HT with organic-sodium dihydrophosphate mobile phases [after [ 49]], c) HVA, 5-HIAA, [ 50] and d) MHPG with organic sodium-acetate mobile phases [ 51].
When antiparallel duplex Tm determinations were run on one LightCycler, the intra-assay coefficient of variation was 0.19%, whereas the inter-assay coefficient of variation was 0.12% (Table 1).
Similar(54)
The determination was run at the Abbott Centre of Excellence research laboratory in Munich, Germany [ 32].
Melting curve determinations on the LightCycler were run in quadruplicate using sodium phosphate buffer, pH 7, for antiparallel duplex and sodium acetate buffer, pH 5, for parallel triplex formation and standard probe concentrations.
Control strains of E. coli (ATCC 25922) and P. aeruginosa (ATCC 27853) were run with each set of MIC determinations.
QCs were run over three independent runs, three independent determinations per QC level.
Both assays were run at 25°C for 30 min. All determinations were carried out in triplicate.
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