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The radioimmunoassay kits for insulin and glucagon determinations were obtained from Diagnostika (Falkenberg, Sweden) and Eurodiagnostica (Malmö, Sweden), respectively.
Samples for bulk density determinations were obtained from the wall of the soil pit in each of the six sampling plots.
Urine samples for 8-OHdG determinations were obtained from 63 healthy adult subjects living in the measured dwellings.
Determinations were obtained from eight animals per group, and the differences were examined using one-way analysis of variance (ANOVA) followed by the Fisher test (Stat View).
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Other samples for cytokine determination were obtained from supernatants of stimulated lymphocyte cultures.
Reagents for protein determination were obtained from the following sources: Bio-Rad protein assay kit, Bio-Rad (Hercules, CA); bicinchoninic acid (BCA) assay, Pierce Chemical Co.
Primers used for adenovirus identification and species determination were obtained from the literature.
All the reagents for GGT determination were obtained from Sigma, Israel.
NOE constraints for the structure determination were obtained from N-edited and homonuclear NOESY obtained on a Bruker Avance600 MHz spectrometer.
Crystals suitable for structure determination were obtained from a construct of the Tiam2 PH CC Ex region that included residues 500 757.
Experimental phases for the initial structure determination were obtained from Single Anomalous Diffraction (SAD) experiments on crystals grown from selenomethionine-derivatized proteins by using the software package PHENIX (Adams et al., 2002).
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