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Duplicate determinations were measured for each paw and averaged.
Protein determinations were measured with SOFTmax software package on an E-max microplate reader (Molecular Devices, Sunnyvale, CA).
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The two-phase pressure drop and the liquid hold-up (liquid RTD determination) were measured for pressures up to 8.1 MPa.
Three-bond homonuclear coupling constants (JHH) used for structure determination were measured from H-1D spectra recorded at 900 MHz (16 384 complex points, dwell-time 92.5 μs) on both D2O and H2O samples.
The influence of blood content on MNP determination was measured by mixed mouse blood and MNPs.
Each lipid determination was measured in triplicate.
The proportion of explained variance (coefficient of determination) was measured by R. We selected 29 candidate genes in the warfarin interactive pathways (Fig. 1 and Supplementary Table S1) and tested them for ∼800 SNPs that comprehensively captured common variation based on measures of LD (r ≥ 0.8).
As for the receptor determinations, mRNAs were measured at only one point of the treatment period, and an earlier increase in mRNA concentrations could have been missed.
After species determination, they were measured and bled by orbital puncture.
Biochemical determinations: routine chemistries were measured using the Vitros® 4600 Chemistry System.
After IC50 and MIC determinations, the MFCs were measured by subculturing the supernatants from the susceptibility testings.
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