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All determinations were done weekly between 0800 and 0900 hours.
Total N determinations were done by the Kjeldahl method of Bremner (1965).
All determinations were done at least in triplicate [see Additional file 2].
Total N determinations were done by the Kjeldahl method of Bremner [29].
Urea determinations were done following the method of Goeyens et al. (1998).
Five separate experimental determinations were done for the natural frequency of each specimen and then the average value was adopted.
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Rigorous determination of specific activity for both free and cross-linked enzymes requires that lipase activity determinations are done in linear regions of conversion-time and activity-protein mass/concentration data.
Secret GRAS determinations are done "to retain confidentiality of your material," McQuate said.
Statistical analysis, graphing and EC50/IC50 (median effective concentration/median inhibitory concentration) determination were done using Graph Pad Prism (ver.5.0a).
Each experiment and every determination were done at least in triplicate, and the levels of MMP7, Fas and FasL were calculated using the ΔΔCt method.
The antibody preparation, purification and antiserum titer determination were done as previously described [ 31].
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