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Blood samples for hormone determinations were collected on Days 40, 90, 120, 150, 180, and 210 of gestation.
Tracheal aspirates for amikacin concentration determinations were collected on day 3 from 19 patients at 69 time points.
The faecal samples for all dogs (n = 5) for calprotectin determinations were collected on the last three consecutive days at the end of each dietary period then pooled and thoroughly mixed.
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Probes (faeces, dust and aerosol probes for MIC determination) were collected on the following days: 0, 5, 8, 14, 21, 28, 34, 37, 42, 50, and 53.
Samples for end-product determinations were collected immediately after the final OD600 reading was taken.
Blood samples for biochemical and hormonal determinations were collected in tubes for serum separation.
Three and sometimes four blood pressure determinations were collected by a physician using a mercury sphygmomanometer.
The blood samples for clonidine determinations were collected in 4 mL heparin tubes.
For hatch rate determination, embryos were collected on apple-agar plates for 4 hr, than the parents were removed and more than 500 embryos per genotype were counted after 2 days' incubation.
For dry weight determinations, cell cultures were collected on tarred membrane filters (type HA, 0.45 um pore size; Millipore Corp).
In order to minimize effects of non-hydrostatic stress on volume and pressure determination, P V T data were collected on cooling cycles from the peak temperature.
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