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Blood samples for hormone determinations were collected on Days 40, 90, 120, 150, 180, and 210 of gestation.
Interventions: Blood samples (2 mL) for S-100 determinations were collected after the induction of anesthesia, 30 minutes after aortic cross-clamping, 1 hour after the termination of CPB, and 5 and 24 hours after the operation.
Blood samples for biochemical and hormonal determinations were collected in tubes for serum separation.
Samples for end-product determinations were collected immediately after the final OD600 reading was taken.
Three and sometimes four blood pressure determinations were collected by a physician using a mercury sphygmomanometer.
The blood samples for clonidine determinations were collected in 4 mL heparin tubes.
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Blood for plasma determinations was collected by cardiac puncture and anticoagulated in the presence of trisodium citrate (3.15% w:v, 1 voL citrate per 9 vol blood).
Aliquots for protein concentration determination were collected before β-mercaptoethanol addition and protein concentration was later determined by the Peterson method [ 32].
Water samples for dissolved oxygen (DO) and biological oxygen demand (BOD) determination were collected separately into 300-ml BOD glass bottles.
Samples for eGFP level determination were collected every second passage.
Milk samples for P4 determination were collected twice weekly during the morning milking.
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