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The mRNA half-life determinations were calculated using the one-phase exponential decay method (22) using GraphPad Prism software (GraphPad Software, San Diego, CA).
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Coefficients of determination were calculated using Excel (Microsoft Corp., Redmond, Washington).
To compare the difference between central tendency of results, the coefficient of determination was calculated using statistical software and an ANOVA test was performed.
Sample size determination was calculated using Power and Sample Size Program (PS) package (version 3.0.43).
The results represent the mean values of the duplicate determinations and were calculated using the SoftMax Pro 4.8 software (Molecular Devices Corporation, Sunnyvale, CA, USA).
Based on the obtained amplification curves, the Ct values (fractional cycle number at threshold), the value of PCR efficiency and the corresponding coefficient of determination (R2) were calculated using LinRegPCR software (Ruijter et al., 2009) for all four samples (IR64C, IR64S, IR64-Sub1C and IR64-Sub1S).
Coefficients of determination (r 2 ) were calculated using Microsoft Excel 2010.
Distance matrices to be used in the Distance-Based OTU and Richness determination algorithm (DOTUR) [21] were calculated using the DNAML option of DNADIST PHYLIPpackagege) available in the greengenes toolbox [45].
The determination of PCR efficiency, threshold cycle (Ct) determination, and RNA starting concentration were calculated using raw fluorescence data using LinRegPCR analysis software [75], [76].
24-hour CV were calculated using two determinations for PPG (2 hour post –lunch and –dinner glucose levels taken two times a week and the PPG following the standard meal during the examination.
Coefficients of determination (R) for the robust regression models were calculated using STATA rregfit command.
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