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Lactate determinations were at the discretion of the attending service and hyperlactatemia was defined by a lactate level > 2 mmol/L.
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However, it is not obvious that legal determinations are at the heart of the matter when we speak of actions that transcend borders in one way or another.
The number of determinations was at least 5 in both experiments.
The criteria for a successful identity determination were: at least 3 matched peptides and 20% sequence coverage; isoelectric point differing by at most 2; and molecular weight differing by at most 20% compared with the experimental estimate.
"Determination was at a higher level, and everybody contribute.
These determinations were performed at baseline and at 3 months.
Samples were immediately frozen at −20°C then stored at −80°C, and all the determinations were done at the same time (2 years after the first inclusion).
Three fluorescence measurements were averaged per determination, and at least three separate determinations were made at each ligand concentration.
The determinations were performed at the Department of Animal and Land Resources, The Agricultural University of Iceland, at Keldnaholt, Reykjavík.
Two independent determinations were conducted at the Oregon Department of Fish and Wildlife (Clackamas, OR) and at University of California Davis (Davis, CA).
The last determinations were made at the onset of the clinical signs or at censoring.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com