Guo To check ploidy levels of the populations studied, two methods, either chromosome counting or DNA ploidy level determination, were applied using young flower buds or fresh or silica gel-dried leaves, respectively.
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After optimization, the PCR procedure for sex determination was applied to 14 embryo biopsies.
Molecular dynamics simulations combined with a variation of the Weighted Histogram Analysis Method for potential of mean force determination are applied to characterize localized structural changes for the test case of cytosine (underlined) base flipping in a GTCAGCGCATGG DNA duplex.
Monitored control and mild water deficit stress (averaged determination) was applied to a segregating T3 population of esk1-6 mutant plants (203 individuals).
For this purpose, the method of calculating the ratio of the obtained results and the uncertainty of the determination was applied.
The DER determination is applied to all reads in the combined non-redundant set from Step 4. For DERs, the median k-mer abundance is calculated for each dataset and the ratio of median k-mer abundance (RoM) provided as a prediction of gene expression ratio.
To determine the effect of different Mn2+ concentrations of PepP activity, standard conditions for PepP activity determination (see above) were applied, except that different concentrations of MnCl2 (0 mM to 14.28 mM in the assay) were tested.
Three evaluation indices, the error percentage of discriminated snowfall days (Ep), the relative error of discriminated snowfall (Re) and the determination coefficient (R2), were applied to assess the discrimination accuracy.
For the statistical analysis, the Pearson (r) and determination (r) coefficients were applied and a P-value <0.05 was considered significant.
