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The means of triplicate determinations were analyzed using t-test with paired comparison for means (18 observations from 9 donors).
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Water and fish samples for heavy metal determination were analyzed using the VARIAN AA240FS Flame Atomic Absorption Spectrometer which is a fast multi-element technique with a dynamic linear range and moderate low detection limits.
Samples for GGT determination were analyzed using the spectrophotometric method described by Genders and Kaplan [ 23].
For determination of plasma norepinephrine, samples were analyzed using a commercially available ELISA kit (Rocky Mountain Diagnostics, Colorado Springs, CO) according to the manufacturer's instructions.
For the determination of S100B, all samples were analyzed using a commercially available immunoluminometric assay (LIASON; Sangtec Medical, Bromma, Sweden).
Both plasma and brain samples were analyzed using LC/MS/MS determination, according to ADME-Bioanalysis (Vergeze, France) procedures.
Results of the histologic evaluation as well as from determination of lavage specimen and real-time PCR were analyzed using one-way ANOVA with posthoc Scheffé.
For determination of differential gene expression, FES derived output data files were analyzed using the Rosetta Resolver gene expression data analysis system (Rosetta Biosoftware).
Firing patterns were analyzed using the method based on the determination of density histogram developed by Kaneoke and Vitek [21] as previously described [18], [22].
Global methylation profiles were analyzed using the HumanMethylation27 BeadChip microarray allowing the determination of DNA methylation levels at 27,578 unique CpG sites within more than 14,000 promoter regions.
Raw material and WIS fraction were analyzed using the standard National Renewable Energy Laboratory (NREL) methods for determination of structural carbohydrates and lignin in biomass [ 39].
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