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All determinations were analysed in serum by using an AU2700 Olympus analyser.
All serum concentrations (triplicate determinations) were analysed in a blinded manner with respect to any clinical information.
The intragroup variation in length of survival, including the S.D. and 95% confidence interval determinations, were analysed.
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Duplicate dishes of cells for each determination were analysed.
Several factors complicating the stress determination are analysed and discussed: (1) ghost stresses arising from a small variation in the shallow information depths probed with GI-XRD, (2) selection of the grain interaction model used to calculate the X-ray elastic constants for conversion of lattice strains into residual stress and (3) the composition dependence of these elastic constants.
Results of the MIC determination by microdilution were analysed with Exact Fischer Test using Microsoft Excel software (Office 2007).
The samples from this solution were analysed for determination of the silicic acid released in the media.
Three samples (S1, S2 and S3) of the same hydrolysate and one sample of gelatine (S0) were analysed for determination of their molecular weight.
Each point in the curve represents the mean of nine determinations (± SEM), differences among conditions were analysed using Student's t-test (p < 0.05).
The capillary blood samples were analysed immediately for determination of pH and bicarbonate (HCO3 −) via an automated analyzer (Radiometer, Brønshøj, Denmark).
Determination of tight-binding ligand characteristics were analysed based on the equation developed by Morrison [29].
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