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Specimen determinations were adjusted accordingly, all genetic distances were recalculated, and the number of haplotypes per species was determined using the DNA barcoding tools available at http://www.ibarcode.org[ 88].
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After 3 months of pump therapy (visit 5), pump and CGM data were downloaded again, blood samples were taken for A1C determination, and treatment guidelines were adjusted as needed.
Samples obtained from the time course of different fractions (cytosol or mitochondria) were adjusted for equal protein loading by determination of concentrations with the Bradford assay.
Estimates were adjusted for the effects of age at BMI determination and for smoking status chronologically closest to the point of BMI determination (current or former smoker vs. never smoker).
Protein concentrations were determined using the DC protein determination kit (BioRAD) according to manufacturer's protocol and samples volumes were adjusted for loading.
Masks were adjusted.
For Os determination the instrument was adjusted for optimum performance standard no gas mode using the supplied autotune protocols.
For Ru determination the instrument was adjusted for optimum performance in He KED (kinetic energy discrimination) mode using the supplied autotune protocols.
The quick increase of the CEC value indicates that the increase of soil pH activates immediately negative charges of the CEC (very weak acids; [39]); nevertheless, the methodology of CEC determination (the soil pH is adjusted to neutrality with ammonium acetate) could partially mask the extent of this increase.
The samples were stored at 4 °C and the solution pH was adjusted before determination.
Any model or models designated under such regulations may be adjusted upon a determination, after notice and opportunity for public hearing, by the Administrator that such adjustment is necessary to take into account unique terrain or meteorological characteristics of an area potentially affected by emissions from a source applying for a permit required under this part.
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