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The average of three consecutive determinations was used to calculate ΔPPrad for statistical analysis.
A solution hybridisation assay for specific mRNA determinations was used and performed as before [ 21].
Determinations were performed in duplicate, and the mean value of both determinations was used.
The mean of these determinations was used to calculate blood pressure z-scores and percentiles relative to a child's sex, age and height [ 35].
They were measured for height and weight, had fasting blood drawn for serum PCBs, pesticides, and serum lipids, and had three independent measures of blood pressure taken at 2-min intervals after having been seated for 5 min. Because no significant differences were found among the different blood pressure measurements, the average of the three determinations was used for analysis.
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Five different determinations were used to develop the statistical data related to calibration curve (Table 1).
For the spectrophotometric analysis, reagent blank determinations were used to correct the instrument readings.
Electrophoretic mobility and optical absorbance determinations were used with this aim.
For each set of thermodilution determinations, the calculated mean values of the determinations were used for haemodynamic management and statistical analysis.
The mean values of the three consecutive determinations were used for statistical analysis.
The mean values of the three determinations were used for statistical analysis.
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