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Sampling for pharmacokinetic determinations was conducted in all patients from each dose level during the first and second cycles of therapy.
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For each run, six determinations were conducted.
For each run, triplicate determinations were conducted.
All determinations were conducted in triplicate and all the results were calculated as Mean ± SD (SD).
A total of 910 analytical determinations were conducted from aliquots of 10 silo ovine milks.
The morphological and phytochemical and antioxidant activities determinations were conducted on the field-grown S. flavescens populations.
In total, 1440 determinations were conducted for each mycoplasma species in milk samples subjected to different storage temperatures (refrigeration at 4 °C or freezing at −20 °C), preservation strategies (no preservative, NP; azidiol, AZ; or bronopol, BR) and storage times at each temperature (0, 2, 4, 6, 8, 10 and 24 h at 4 °C and 48 h, 1 week, 2 weeks and 4 weeks at −20 °C).
Allele size determinations were conducted twice to verify accurate scoring and are reported according to their true allele size by excluding the 5' nucleotides of the fluorescent-labeled M13 primers.
Hormone determinations were conducted at the ILAT Steroid RIA Laboratory of the University of Massachusetts Medical School.
All determinations were conducted in accordance with manufacturers' protocols.
All CAS measurements were performed in triplicate, and at least two independent determinations were conducted.
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