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Blood for plasma determinations was collected by cardiac puncture and anticoagulated in the presence of trisodium citrate (3.15% w:v, 1 voL citrate per 9 vol blood).
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Blood samples for hormone determinations were collected on Days 40, 90, 120, 150, 180, and 210 of gestation.
Interventions: Blood samples (2 mL) for S-100 determinations were collected after the induction of anesthesia, 30 minutes after aortic cross-clamping, 1 hour after the termination of CPB, and 5 and 24 hours after the operation.
Samples for end-product determinations were collected immediately after the final OD600 reading was taken.
Blood samples for biochemical and hormonal determinations were collected in tubes for serum separation.
Three and sometimes four blood pressure determinations were collected by a physician using a mercury sphygmomanometer.
The blood samples for clonidine determinations were collected in 4 mL heparin tubes.
Tracheal aspirates for amikacin concentration determinations were collected on day 3 from 19 patients at 69 time points.
Blood samples for baseline determinations were collected from each patient after inclusion and just prior to initiating paricalcitol treatment.
Blood samples for serum EPO concentration determinations were collected by venipuncture, before and 2, 6, 10, 14, 17, 20, 24, 48, 72, 96, and 120 hours after injection.
Fifteen determinations were collected in 13 patients in septic, cardiogenic and hypovolemic shock (males 9/13, age 73.2 ± 5.8, ejection fraction 54% ± 8).
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