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Duplicate determinations using three different solvent ratios were performed for each complex.
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All reported values are averages of at least three independent determinations using nine PLM concentrations.
Experiment 1 was a nutritive value determination using four crossbred steers and four diets in a 4×4 Latin square design experiment.
In this study three different aggregate gradation as per Ministry of Road Transport and Highways (MoRT&H), India, were evaluated for fatigue life determination using four point beam bending (4PBB) test.
DOI: http://dx.doi.org/10.7554/eLife.04106.006 10.7554/eLiFigure06.007 Figure 1 figure supplement 3. Reproducibility and coverage threshold determination using two fully independent biological replicates.
Here, we introduce volume determination using two standards (VDTS) as a general quantitative method for the analysis of polar metabolites in submicrolitre samples using H NMR spectroscopy.
We obtained the extraction volume by indirect means, as described in schematic form in Figure 2. We denote this procedure as "volume determination using two standards" or VDTS.
We select Drosophila larval hemolymph as a challenging test case and introduce the method of volume determination using two standards (VDTS) to assess accurately the sample dilution and original analyte volume.
End-point turbidity measurements were recorded after 48 h of growth, and all MIC determinations were performed using three biological replicates.
The determination was carried out using three replicates at each concentration level.
Numerical hypocenter determination tests using four virtual hypocenters, the mainshock, the largest aftershock, a point 100 km north of the array, and a point just beneath the array, were carried out.
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