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Protein determinations used the Coomassie Plus Reagent (Pierce, Rockford, IL).
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Cardiac output determinations using the LiDCO™ system are very well correlated with Pulmonary Arterial ThermoDilution measurements [108] as well as with values obtained using the PICCO™ system [103].
Because of the variation in IBC recorded in BR-preserved samples, we recommend that BR should not be used for TBC determinations using the automated flow cytometry method.
The surface characteristics of the MIP and NIP (control polymer) samples were evaluated by means of surface area and pore volume determinations, using the BET method.
In addition, it is better to correlate the two determinations using the same fusion protein.
Information regarding STEM data analysis and mass determinations using the program PCMass are described at http://www.bnl.gov/biology/stem/Data_Analysis.asp and in [24] and [25].
Furthermore, the K m for bd-I was almost 100-fold higher than previous determinations using the globin deoxygenation method [32] (Table 1).
Data were analyzed in multiple samples after n = 3 determinations using the SPSS software package (SPSS Inc., Chicago, IL, USA) and, where appropriate, are expressed as means ± sd.
Insulin sensitivity was calculated from fasting serum insulin and plasma glucose determinations using the homeostasis model assessment of insulin resistance (HOMA-IR) method.
Pulmonary function determinations using the forced oscillation technique and PV curve analysis were easy and rapid and did not compromise subsequent biochemical assays.
The formula for sample size determination used the prevalence of non-adherence to anti-malarial and this was estimated at 50% since the prevalence is not known.
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