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5) Micronutrient determinations: The levels of 11 different micronutrients, including Zn and Fe were measured in the mature, harvested grain using ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) (Agilent Technologies, Santa Clara, CA, USA) at the University of California, Davis.
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Determinations of the levels of CA 125 were performed with the Bayer Immuno ITM CA-125 II assay.
Precise determination of the levels of heteroplasmy is crucial since the level of heteroplasmy determines both the penetrance and severity of expression of some mitochondrial diseases.
These tests include determination of the levels of the blood glucose, blood urea nitrogen, and serum protein.
Moreover, determination of the levels of plasma progesterone, estradiol, FSH and LH was also made.
Determination of the levels of each factor.
Every cDNA sample was tested in triplicate and the obtained crossing point (Cp) value facilitated the determination of the levels of starting message using a specific standard curve.
An alternative method for evaluating angiogenic activity is the determination of the levels of each angiogenic factor in tumour tissues and serum (Poon et al, 2001).
Determination of the levels of admixture across the BAPS clusters based on MLST data (fig. 3) supports the idea of discrete clusters but with significant recombination.
Thus, in the early stage of SIRS before the occurrence of AKI, determination of the levels of presepsin might be an objective parameter before clinical diagnosis of AKI.
For determination of the levels of pro-inflammatory cytokines, total RNA was isolated from cells using TRIzol (Invitrogen) following the manufacturer's protocol.
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