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Higher removal percentages for the most hydrophobic surfaces were expected since, as it was stated in the 'Surface parameters and hydrophobicity determinations' section, when ( varDelta {G}_{sws}^{mathrm{TOT}} ) decreases, the interaction between the two entities is stronger than the interaction of each entity with water.
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Doubling time was determined as described in the Doubling Time Determination section of Materials and Methods except cultures were grown in SCD -URA) inSCD -URA YPAD to ensure plasminsteadtenance.
Total protein concentration was determined by the bicinchonic acid method, as mentioned in the Protein determination section above.
Our algorithm consists of the following main steps: Section 2.1 threshold determination; Section 2.2 voxel classification; Section 2.3 density-based clustering; Section 2.4 black voxel inclusion.
As mentioned earlier in the CMC determination section, a turning point in the plot of electrical conductivity against surfactant concentration represents the CMC of the surfactant.
Serum samples used for sensitivity and specificity determination (section 2.8) were preadsorbed with pellet, heat denatured and non-heat-denatured cell lysates of only E. coli XL1-Blue MRF'.
The esterification activity of immobilized lipase, prepared as described in the Lipase activity determination section, was 150 Ue per 1 g of membrane.
"Grasping position detection" and "Grasping motion determination" sections explains the proposed method.
For microvessel density determination, sections were incubated with polyclonal antibody against human Factor VIII-related antigen (Dako Corporation) at a 1 : 300 dilution in PBS containing 10% goat serum for 30 min at room temperature.
For both VEGF and TS determinations, each section was scored by two separate observers (FDP, AMG) at light-microscope (200× and 400×) and, in cases when the difference exceeded 10%, by a third observer.
Therefore, the spectra S are acquired within a path length window Δℓ, which determines the spatial resolution for the µ t-determination (see Section 2.3).
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