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Standard determinations of total cholesterol and serum albumin concentrations were determined by using commercialy available kits suitable for use on Olympus 2700 instrumentation (Olympus, Milano, Italy).
All data points on each binding curve were performed in triplicate and each 96-well plate also contained 3 6 determinations of total and non-specific binding.
Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins.
Reference determinations of total Hb were performed by hospital laboratory using clinical photometric HiCN analyzer XT-2000i/XT XT-2000i/XTpan).
For competition binding, all data points on each binding curve were performed in triplicate, and each 96-well plate also contained 6 determinations of total and nonspecific binding.
This observed variability in AAG levels may also complicate the interpretation of pharmacokinetic results when based only on determinations of total drug concentrations.
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Total sulfurs of the samples were determined according to ISO 351 1996 (Determination of total sulfur-High temperature combustion method).
Determination of total bilirubin was determined by the method described by [ 75].
Total moisture was determined following ISO 589 2003 (Hard coal- determination of total moisture).
The determination of total phenolic contents in ceSb was determined by the Folin Ciocalteu method with slightly modifications.
Standard Test Method for Determination of Total Knee Replacement).
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