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For the determinations of lipids the liver and kidney tissues were weighed and lipids were extracted from tissues by the method of Folch et al [ 22] using chloroform – methanol mixture (CHCl3: MeOH)(2 1 v/v).
Blood was drawn after 12-h fasting from the antecubital vein in the morning for determinations of lipids (total cholesterol, LDL cholesterol, triglycerides, and HDL cholesterol), plasma glucose, insulin, HbA1c, blood urea nitrogen, creatinine, uric acid, and high-sensitivity C-reactive protein (hsCRP).
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As a fourth objective, we made determinations of lipid peroxidation.
Within 24 h of neuropsychological testing, blood samples were drawn from each participant after an overnight fasting period for determinations of lipid profile and fasting plasma glucose and insulin.
Most clinicians must rely solely on the quantitative determination of lipids.
We performed a cross-sectional analysis of the role of endogenous testosterone (T) and estradiol (E2), as well as their respective biologically active fractions, in the determination of lipids and lipoproteins in an occupation-based cohort of 715 healthy middle-aged men.
At baseline, blood samples were collected for determination of lipids, high sensitivity C-reactive protein (hsCRP), HbA1c, hyaluronan and hyaluronidase.
Blood sample analyses included determination of lipids (cholesterol, HDL-cholesterol, triglycerides, LDL-cholesterol, oxidized lipoxidizeds) and other biolipoproteinsers (IGF, IGFBp-3, C-peptide, leptin, andpotherin, para-oxonase, insulin) (Tabiological
Total fat content and fatty acid composition were determined according to Duckett et al. 8 For determination of lipid fractions, total lipids were extracted from ST muscle samples via Folch et al 10 method.
These advances allow the determination of lipid profile alterations and how these are associated with diseases.
Care should be taken that the correct lipid extraction procedures are used for the determination of lipid content in fish samples from bioaccumulation studies [14].
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