For the determinations of lipids the liver and kidney tissues were weighed and lipids were extracted from tissues by the method of Folch et al [ 22] using chloroform – methanol mixture (CHCl3: MeOH)(2 1 v/v).
Blood was drawn after 12-h fasting from the antecubital vein in the morning for determinations of lipids (total cholesterol, LDL cholesterol, triglycerides, and HDL cholesterol), plasma glucose, insulin, HbA1c, blood urea nitrogen, creatinine, uric acid, and high-sensitivity C-reactive protein (hsCRP).
Total fat content and fatty acid composition were determined according to Duckett et al. 8 For determination of lipid fractions, total lipids were extracted from ST muscle samples via Folch et al 10 method.
These advances allow the determination of lipid profile alterations and how these are associated with diseases.
Care should be taken that the correct lipid extraction procedures are used for the determination of lipid content in fish samples from bioaccumulation studies [14].
Phosphine reagents were designed and synthesized as a new type of fluorescent reagents for the determination of lipid hydroperoxides in foodstuff and biological materials.
Each method currently employed for the determination of lipid content has its advantages and disadvantages, and there is no procedure available which is suitable for all types of lipids.
In conclusion, the results of our survey indicate that analytical methods commonly applied by governmental, academic, and industrial labs for the determination of lipid content in fish sampled from bioaccumulation studies are of high quality.
However, the choice of method used for the determination of lipid content will be dictated to a large extent by the cost and by the urgency with which the results are required.
