Our results indicated that ClCOBL1 was involved in the determination of leaf dorsoventrality and anisotropic expansion possibly by affecting the expression of adaxial and abaxial identity genes.
Recent investigation of BOP activity in a variety of land plants provides a more complete picture of their conserved functions at lateral organ boundaries in the determination of leaf, flower, inflorescence, and root nodule architecture.
We have investigated endogenous tasiRNAs from the TAS3 loci, which function in the determination of leaf shape in Arabidopsis thaliana [3], [4] and also in maize where different factors involved in tasiRNA biosynthesis have been localized in a polar fashion in leaf primordia [14], [15].
Interestingly, the RIC1-mediated MT organization in turn suppresses ROP2 activation in the indentation zone, leading to the suppression of RIC4 which promotes AF formation in the regions of growing lobes and the ultimate determination of leaf epidermal cell shape [23].
The exact determination of leaf area is nearly impossible.
ImageJ macro for determination of leaf area and green leaf area.
Extraction and determination of leaf MDA was performed according to Hodges et al. [ 115].
The penultimate leaves from five tillers were sampled at mid-grain fill for determination of leaf B concentration (PDF 464 kb) Additional file 8: Table S5.
A number of genes known to affect meristomatic pattern formation (e.g. AS1 and WUS, KNOX and CLV, see [ 1] for a review of leaf development), the rate of leaf primordia initiation [ 2] and that contribute to the determination of leaf length (ROT3 [ 3], LNG [ 4]) and width (AN [ 5]) have now been identified: less is known about the determination of leaf size currently.
There are genes known to affect meristomatic pattern formation (e.g. AS1 and WUS, KNOX and CLV, see [ 2] for a review of leaf development) and the rate of leaf primordia initiation [ 3] as well as genes that contribute to the determination of leaf length (ROT3 [ 4], LNG [ 5]) and width (AN, [ 6]), with less being known about the determination of leaf size (although [ 7] provide exciting insight).
