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Determinations of insulin sensitivity and β-cell function were made on admission only.
GTTs, ITTs and PTTs, as well as determinations of insulin levels, were performed as described previously [ 34], except that male CD1 mice were used instead of rats.
Serum was aliquoted and frozen at -80°C for further determinations of insulin, GH, IGF-I, IGF-BP3, FGF-2, EGF and PDGF-B concentrations by ELISA (Peprotech Paris, France).
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Total insulin was extracted with 0.18 M HCl in 70% ethanol for determination of insulin content.
A silica gel-modified carbon paste electrode (Si-CPE) was fabricated and used for selective and sensitive determination of insulin.
Pencil graphite electrode modified by CS@Z was applied for electrochemical ultra-sensitive determination of insulin.
Therefore, the modified electrode was used as an electrochemical sensor for the differential pulse adsorptive stripping voltammetric and hydrodynamic amperometric determination of insulin.
At 10 weeks, a biopsy of the skeletal muscle was performed for determination of insulin receptor, PPARα, and PPARγ content.
The results demonstrated that the sensor can be used for the determination of insulin in the commercially available injections.
An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy.
The novel magnetically controlled sensing membrane showed good selectivity and reproducibility for the determination of insulin with the detection limit reaching 17 pM (S/N = 3), and this limit of detection was further reduced to 3 pM by using the Fe CN 63−/4− redox probe.
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