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Specific primers for the determinations of gene dosage, allelic frequency (GD1) and chromosome rearrangement boundaries.
Another source of mis-estimation of gene dosage might result from the combination of hemizygosis and non-specific cross-reaction of the primers, as presented in Figure 3F that originate altered determinations of gene dosage in the resulting haploid genotypes.
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Gene knockout is the most powerful tool for determination of gene function or permanent modification of the phenotypic characteristics of an animal.
In addition, array-based approaches allow the determination of gene expression changes that accompany the progression to acute leukemia.
Robust droplet-based, single-cell simultaneous determination of gene expression and immune repertoire indicated that PDO TILs accurately preserved the original tumor Tcell receptor (TCR) spectrum.
This will strongly complement an iterative approach to the evaluation of capsid variants in the clinic and, should wherever possible, include the determination of gene transfer efficiencies.
Nuclear medicine procedures may be applied for the determination of gene function and regulation using established and new tracers, or using in vivo reporter genes, such as genes encoding enzymes, receptors, antigens, or transporters.
The identity was additionally confirmed by phage-typing, by determination of the size and the number of repeats of the X region of spa gene, by determination of gene polymorphisms of coa gene and by macrorestriction analysis of the chromosomal DNA of the isolates by pulsed-field gel electrophoresis.
Cells were collected for the determination of gene expression levels and then used in bioconversion using resting cells for another period of 2 days.
cDNA was used for determination of gene expression level using TaqMan Low Density Arrays (Applied Biosystems).
The standard curves for the determination of gene numbers were established in the same manner described previously [47], [48].
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