The aim of the determination of diversity was to present endophytes from various beneficial plant species (fruit, ornamentals, weeds, medicinal plants).
In the determination of species diversity, the Shannon-Wiener diversity index as described by Krebs (1989) was used.
Selecting target genomic regions to serve as proxy for the full-length genome is a popular approach for studies of fungal diversity, as it is in the determination of bacterial diversity in microbiome studies.
Built upon a series of bioinformatic tools, both pipelines allow: the trimming, screening, and alignment of sequences; the assignment of operational taxonomic units (OTUs); phylogenetic analyses; and determination of fungal diversity within and across groups (referred to as α and β diversities) [ 29, 30].
Glanville, J. et al. Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire.
Lee, J. H., Kim, T. W., Lee, H., Chang, H. C. & Kim, H. Y. Determination of microbial diversity in meju, fermented cooked soya beans, using nested PCR-denaturing gradient gel electrophoresis.
Radomski, N., Thibault, V. C. & Karoui, C. Determination of genotypic diversity of Mycobacterium avium subspecies from human and animal origins by mycobacterial interspersed repetitive-unit-variable-number tandemrepeat and IS1311 restriction fragment length polymorphism typing methods.
Twenty-two genomic SSR markers were tested in 39 asparagus accessions belonging to ten cultivars and one accession of Asparagus setaceus for determination of genetic diversity.
Furthermore, the novel mesocosm system can be employed for advanced antifouling analysis including metagenomic approaches for determination of microbial diversity attaching to different coatings under changing shear forces.
These findings indicated that the type-specific PCR assays described herein are useful for the determination of genotypic diversity of the B. bovis msa-1 gene in Asia.
