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PCR analysis of this region computed a copy number of 4.18+/- 0.45 and 4.47+/-0.58 reinforcing the sensitivity of copy number determinations obtained using the VN approach.
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In another paper, Martini et al. [40] compared SFC determination obtained using conventional offline NMR instrument with determination obtained by single-sided NMR sensor.
Cr-EDTA clearance was measured for 90 min pre-CPB and then at 90 min and 24 hours post CPB using single bolus Cr-EDTA injections with clearance determination obtained using the slope intercept method as previously described [ 13].
The relative level of expression of the TbMyo1 transcript was investigated using qRT-PCR and relative expression ratios (bloodstream/procyclic form) of 2.8±0.3 and 1.7±0.5 (both mean ± SD of three determinations) were obtained using total RNA and purified mRNA fractions respectively.
The P(A a O2 gradient was calculated according to the following formula [ 2, 10]: P(A a O2 = [150–(1.25 × PaCO2)]–PaO2 The arterial bood gas determinations were obtained using IL 1650 blood gas analyzer (Instrumentation Laboratory, Milano, Italy).
Crystals suitable for structure determination were obtained using microbatch methods by mixing equal volumes (2 μl) of 15 mg/ml RII tetra-tandemer with the same precipitant solution as above, followed by the addition of 0.5 μl of 5% (w/v) n-Octyl-β D-glucoside.
However, there are limited studies on the sample size determination of speeds obtained using video-graphic survey.
ACIS provides a sensitive, efficient and highly reproducible method of DNA ploidy determination, and results obtained using this system correlated well with those obtained by the CAS 200.
Samples Reported value (μg g-1) Found by proposed method (μgg-1)a Multivitamin Cu: 3.98 Cu:3.95 ± 0.13 Infant milk substitute Cu 2.90 Cu: 2.89 ± 0.25 Hydrogenated oil Ni: 4.50 Ni:4.3 ± 0.3 (N = 5) a Mean of five determinations ± standard deviation Table 6 Results for metal ions determination in food samples obtained using the optimum conditions.
For Microcoleus sp. biomass determination, the binary stacks were obtained using the same optimum threshold as in total biomass determination.
Table 3 Results for metal ions determination in certified reference samples obtained using the optimum conditions.
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