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A549-NT cells had a doubling time of approximately 24 hours and A549-T cells of approximately 20 hours as determined by cell cycle determinations (data not shown).
The same behaviour was detected in the regional CBF determinations (data not shown).
Despite extensive testing we were not able to detect any changes in FRET efficiencies on addition of the NO-donor DEA/NO in concentrations that led to activation in enzyme activity determinations (data not shown).
In addition, despite the different levels at 24 and 48 h of co-cultures, each mediator showed a trend and a significant difference in both determinations (data not shown).
To measure 5-mC and 5-hmC in the AWHS, we used an ELISA method (5-mC and 5-hmC kits; Zymo Research) following the manufacturer's instructions; we confirmed that the results were consistent in a subsample of the SHS with duplicate determinations (data not shown).
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These data can be included with the antigen intensity to score cells as cancerous or not, or in some cases can be used as a single parameter for this determination (data not shown).
Since whole spleen samples were used, a low background of contaminating sequences from normal B cells was observed but these mixed signals were below the threshold allowing individual sequence determination (data not shown).
Typical anthropological methods (e.g. pelvic bone, skull characteristics) and DNA typing were used for sex determination (data not shown).
The resulted mutants have been successfully used to express the proteins for structure determination (data not shown).
Unidentified ticks were conclusively identified by molecular methods developed at UNTHSC, which used amplification of 12S rDNA (Table 1) and sequence determination (data not shown).
Exposure of RAW 264.7 cells, a murine macrophage cell line, to ox-LDL for 24 h resulted in a marked accumulation of intracellular cholesterol, indicated by Oil-Red O staining and HPLC determination (data not shown), confirming the formation of foam cells.
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