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A549-NT cells had a doubling time of approximately 24 hours and A549-T cells of approximately 20 hours as determined by cell cycle determinations (data not shown).
The same behaviour was detected in the regional CBF determinations (data not shown).
Despite extensive testing we were not able to detect any changes in FRET efficiencies on addition of the NO-donor DEA/NO in concentrations that led to activation in enzyme activity determinations (data not shown).
The data are presented as mean (standard deviation of three determinations (data are not shown).
In addition, despite the different levels at 24 and 48 h of co-cultures, each mediator showed a trend and a significant difference in both determinations (data not shown).
To measure 5-mC and 5-hmC in the AWHS, we used an ELISA method (5-mC and 5-hmC kits; Zymo Research) following the manufacturer's instructions; we confirmed that the results were consistent in a subsample of the SHS with duplicate determinations (data not shown).
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As a result, relying on claims or disability determination data could leave out individuals who are unable to work due to a disability.
In this work we studied the concentrations of 0.138 mM, 0.690 mM, 1.380 mM, 3.450 mM and 6.900 mM (determination data would be effected when the concentration is higher than 6.900 mM).
The SSA's 831 file is the official disability determination data used by the Disability Determination Services.
The framework from Kass is missing concrete issues that are specific for DDD, e.g. data security, informational self-determination, data privacy.
This benefit from the definition of cooperative data stream and opportunistic cooperative platform, which could obtain the optimal candidate data packet based on the data packet determination, data length, and user requirements.
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CEO of Professional Science Editing for Scientists @ prosciediting.com