The crystallization reagent that produced the PaMpl crystal for structure determination consisted of 0.2 M sodium acetate, 30% PEG-4000, and 0.1 M Tris-HCl, pH 8.5.
The process of HDI determination consists of choosing upper and lower parameters for each indicator and the normalization is given by an equation that measures the distance between the observed value for the indicator and the minimum value as a proportion of the distance between the maximum and minimum value where I = (observed value-minimum value)/(maximum value-minimum value).
The population for MTD determination (the dose-determining set) consisted of patients treated for ≥21 days in Cycle 1, or who discontinued earlier due to a DLT.
Briefly, OXC determination consisted in the application of a three-potential waveform: (1) at +1.7 V/200 ms, generator potential pulse for oxidation of OXC; (2) at −1.1 V/30 ms, collector potential pulse for reduction of the generated product and OXC quantification; (3) at −1.3 V/300 ms, potential pulse for cleaning the BDDE.
The experimental work consisted of determinations of thin layer drying-toasting curves of soaked soybeans in a fluidised-bed dryer at air temperatures between 100 and 160 °C, for 60 min, using an air velocity of 2.5 m/s.
According to recommendations of a consensus committee [ 14], the image analysis consisted of determinations of (a) flow: proportion of perfused vessels (PPV) and microvascular flow index (MFI); (b) density: total vascular density (TVD) and perfused vascular density (PVD); and (c) heterogeneity: MFI heterogeneity (Het MFI).
The sensation thresholds for the two electrode types were determined using a psychophysical threshold determination method consisting of multiple series of ascending stimuli.
Mechanical testing consisted of determination of Mechanical Detection Threshold (MDT), Vibration Detection Threshold (VDT), Mechanical Pain Threshold (MPT), Mechanical Pain Sensitivity (MPS), Wind-up Ratio (WUR) and Pressure Pain Threshold (PPT).
The substrate for the determination of REH activities consisted of RP and phosphatidylcholine (molar ratio 1 0.9) in 0.1 M potassium phosphate buffer (pH 7.0).
The primary end points for the induction chemotherapy regimens consisted of determination of clinical and particularly pathological response rates, as reported previously (Belembaogo et al, 1992; Van Praagh et al, 1995, 2001; Chollet et al, 1997, 2000).
