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Only three determinations conducted for each group in both immunohistochemistry and Western blotting analyses were based on several previous studies [ 48, 51– 51].
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Sadly, training local troops also did not change the dynamics of an internal struggle for self-determination conducted as guerrilla warfare.
By means of an input output curve determination conducted before every experiment, the largest obtainable fEPSP was found for each individual animal (maximum intensity used 125 μA).
If such non-integrated viral DNA was abundant in cell cultures transduced with the d2 × 250 bp and the 1.2 kb.CMV vectors, titer determination using real-time PCR 3 days after transduction would overestimate the titers, compared to titer determination conducted 7 days post-transduction, where non-integrated viral DNA will be more diluted due to ongoing cell division.
For each run, six determinations were conducted.
For each run, triplicate determinations were conducted.
All determinations were conducted in triplicate and all the results were calculated as Mean ± SD (SD).
A total of 910 analytical determinations were conducted from aliquots of 10 silo ovine milks.
The morphological and phytochemical and antioxidant activities determinations were conducted on the field-grown S. flavescens populations.
In total, 1440 determinations were conducted for each mycoplasma species in milk samples subjected to different storage temperatures (refrigeration at 4 °C or freezing at −20 °C), preservation strategies (no preservative, NP; azidiol, AZ; or bronopol, BR) and storage times at each temperature (0, 2, 4, 6, 8, 10 and 24 h at 4 °C and 48 h, 1 week, 2 weeks and 4 weeks at −20 °C).
Allele size determinations were conducted twice to verify accurate scoring and are reported according to their true allele size by excluding the 5' nucleotides of the fluorescent-labeled M13 primers.
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