Four sets of data contributed to the structure determination, collected from crystals in 5 mM KAu CN 2, 1 mM dimercuriacetate (DMA), 3 mM Au(NO3 4, and no adduct, respectively (Table 2).
Blood samples for baseline determinations were collected from each patient after inclusion and just prior to initiating paricalcitol treatment.
Plasma glucose was clamped at 100 mg/dL (5.5 mmol/L) by a variable rate infusion of 20% dextrose in water, and arterialized blood samples for glucose and insulin determinations were collected from a heated hand vein.
A standard OGTT was also performed on the same day over the 0700 1130 period, and blood samples for glucose determinations were collected from the antecubital vein into a vacuum tube containing sodium fluoride.
Serum for anti-AMA-1 antibody determination was collected from each volunteer at Day 0, 14, 28, 42, 56, 70, 93, 114 and 156 and frozen at −20°C or below until tested as described previously [16].
From 2008 to 2011, reproductive organs (ovaries and uteri), and mandibles (for age determination) were collected from hunter-harvested female moose.
A sample of blood for ploidy determination was collected from each individual by puncture of the caudal vessel using a heparinized syringe.
For example, 50-μL blood samples for plasma glucose determination were collected from the orbital sinus in alloxan-diabetic mice (Ajabnoor and Tilmisany 1988), and 25-μL blood samples for OGTT were collected from the orbital sinus in db/db diabetic mice (Gibbs et al. 1995).
New age determinations on nine samples collected from Bîr Sahara East and Bîr Tirfawi are presented for the five paleolake phases originally described by the excavation team.
