All data are presented as mean ± s.e.m. of triplicate determinations (except saturation binding experiments where determinations were from quadruplicate wells) and n in the text refers to the number of separate experiments.
Three slides were made from each dilution and each was analyzed by immunofluorescence to determine the number of Chlamydia EB/IFU in each dilution; the murine anti-major outer membrane protein (MOMP) monoclonal antibody used in these determinations was from the Micro-Trak system (Syva Corp ,Palo Alto, CA, USA).
The linear range of this biosensor for H2O2 determination was from 5.0 × 10−6 to 1.5 × 10−4 M while its detection limit, based on a signal-to-noise ratio of 3, was 1.3 × 10−6 M.
Enzyme linked immunosorbent assay (ELISA) kits for cytokine determination were from BD Biosciences (San Diego, CA).
The n determinations are derived from different cells, expressing different amounts of protein from the same batch of MEF cells on different days, and throughout the study, cells from different experiments were pooled and are displayed as n determinations.
The radiocarbon age determinations are produced from extracting calcium oxalate contained within mineral crusts associated with the rock art.
The US Fish and Wildlife Service USFWSS) makes determinations for domestic endangered species; these determinations are distinct from the better-known IUCN Red List, which is an international organization.
The subcooled liquid vapour pressure (pL) is one such property, where experimental determinations are limited and far from consistent [1].
All determinations were made from kits originating from the same lot.
The probes used for oERα and oPR mRNA determinations were derived from plasmids containing 360 or 314 bp cDNAs from the ovine ER and PR, respectively, and were kindly supplied by Dr. N. Ing, Texas University, TX, USA [ 25].
