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Rigorous determination of specific activity for both free and cross-linked enzymes requires that lipase activity determinations are done in linear regions of conversion-time and activity-protein mass/concentration data.
Secret GRAS determinations are done "to retain confidentiality of your material," McQuate said.
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All determinations were done weekly between 0800 and 0900 hours.
All determinations were done at least in triplicate [see Additional file 2].
Total N determinations were done by the Kjeldahl method of Bremner [29].
Urea determinations were done following the method of Goeyens et al. (1998).
Total N determinations were done by the Kjeldahl method of Bremner (1965).
Five separate experimental determinations were done for the natural frequency of each specimen and then the average value was adopted.
The salt content determinations were done using a Ionic chromatograph Compact IC Metrohm equipped with a 250 mm Dionex column AS22 and a 50 mm Dionex precolumn AG22.
The height, h, and radius, r, of the conical heap formed were measured and then the angle of repose, θ, was calculated from Equation 7. Determinations were done in triplicate, and the mean was taken.
Size determinations were done by dissolving carbohydrate samples at 3 g/L in 0.1 M sodium acetate, pH 6, with 0.02 % (w/v) sodium azide followed by filtering with a 0.22 µm filter.
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