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The hydro-ethanol extracts for total polyphenol contents determination were prepared as following: 1 g feed samples, grounded and sieved at 0.3 mm was mixed with 20 mL 50% ethanol (v/v).

Micelle solutions for size determination were prepared by the dissolution/evaporation method.

All solutions for the cmc determination were prepared in demineralized double distilled water.

Prior to SDS-PAGE gel analysis, samples (10 μg/lane) used for Oxyblot determination were prepared as per the manufacturer's instructions (Oxyblot Kit, Chemicon, Temecula, CA, USA).

For GSH determination, homogenates were prepared in PEN buffer without reducing agents and were processed immediately as described below.

At 8 and 24 weeks diabetes, the synthesis and purification of methylglyoxal (MG), as well as the synthesis of the derivatising agent and the standards for determination of MG, were prepared according to published procedures [ 14].

For the phase determination, derivative crystals were prepared by quick-soaking native crystals in potassium iodide (KI) solutions prepared in cryoprotection solution for 1 5 min and data collected at the K-edge of 1.55 Å. Diffraction data were processed and scaled with XDS (Kabsch, 2010).

For β-galactosidase activity determinations, crude extracts were prepared with glass beads as described above and 2 mg/ml ONPG (o-nitrophenyl β- D-galactopyranoside) was used as a substrate [ 25].

After rHLB determination, basil oil creams were prepared with two different combinations of surfactants, namely, GMS : Tween 80 (1 : 3.45) and SLS : GMS (1 : 3.68), and evaluated for in vitro antimicrobial activity, skin irritation test, viscosity and consistency.

For NMR structure determination, the following complexes were prepared at concentrations of 1.0 2.2 mM in NMR buffer supplemented with 5 mM MgCl2: SLIds2/N-SLV, SLIds2/CN-SLV, N-SLIds2/SLV, CN-SLIds2/SLV, CN-SLIds1/SLV, N-SLIsbΔIa/N-SLV, SLIsbΔIa/N-SLV, and SLIsbΔIa/CN-SLV.