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The single molecule assay and data processing were performed by J.J. ITC assay, BisChIP-seq assay and data analysis were performed by J.J. and T.L. Celuciferaseuciferassayssay, crystallization and structure determination were performed by T.L. Whole genome methylome data analysis was performed by K.Y. and J.J.
Phase analysis and structural determination were performed using Fullprof software [21] based on the Rietveld method [22, 23].
Elemental analysis, atomic absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis, and surface/interfacial tension determination were performed to investigate the properties of the catalyst.
The addition of substrate, OD measurement, and titer determination were performed as described above.
Cell culture, virus propagation and virus titer determination were performed as described previously [31], [32].
ROC curve analysis and cut-point determination were performed as above for the biomarker score.
Similar(29)
Accurate disulfide bond determination was performed as previously described with slight modifications21.
The fluorometric determination was performed at 483/280 nm.
Primary islets were isolated from ICR mice, KKAy mice and human donor and GSIS determination was performed as previously described51.
Melanin determination was performed by standard Fontana-Masson staining (Ab1506669, Abcam, Oxford, UK) according to manufacturer instructions.
Each determination was performed in triplicate.
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