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From 2008 to 2011, reproductive organs (ovaries and uteri), and mandibles (for age determination) were collected from hunter-harvested female moose.
For example, 50-μL blood samples for plasma glucose determination were collected from the orbital sinus in alloxan-diabetic mice (Ajabnoor and Tilmisany 1988), and 25-μL blood samples for OGTT were collected from the orbital sinus in db/db diabetic mice (Gibbs et al. 1995).
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Serum for anti-AMA-1 antibody determination was collected from each volunteer at Day 0, 14, 28, 42, 56, 70, 93, 114 and 156 and frozen at −20°C or below until tested as described previously [16].
A sample of blood for ploidy determination was collected from each individual by puncture of the caudal vessel using a heparinized syringe.
Blood samples for baseline determinations were collected from each patient after inclusion and just prior to initiating paricalcitol treatment.
Plasma glucose was clamped at 100 mg/dL (5.5 mmol/L) by a variable rate infusion of 20% dextrose in water, and arterialized blood samples for glucose and insulin determinations were collected from a heated hand vein.
A standard OGTT was also performed on the same day over the 0700 1130 period, and blood samples for glucose determinations were collected from the antecubital vein into a vacuum tube containing sodium fluoride.
Samples for eGFP level determination were collected every second passage.
Flower buds of approximately 5 mm in diameter, which represents a critical stage of cucumber sex determination [ 60], were collected from both lines and immediately frozen in liquid nitrogen.
Since radiation damage can be reduced in cryo-temperatures [ 56] and the preserved samples can maintain their close to native structures, the data sets for structural determination are collected mainly from cryo-samples, if possible.
One cricket was collected from each of the six cages for isotopic determination and two crickets were collected from each cage for careful gravimetric hydration measurement.
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